ISO 23349-2020 pdf free.Animal and vegetable fats and oils —Determination of sterols and stanolsin foods and dietary supplementscontaining added phytosterols.
1.This document specifies a reference method for the determination of free sterols/stanols and steryl/stanol esters (0,1 % to 97 % mass fraction) in foods and dietary supplements containing addedphytosterols and in phytosterol food additive concentrates.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of thisdocument.This method does not apply to matrices that contain rice bran oil at concentrations of morethan 20 % due to possible interferences in the gas chromatogram.
2.Normative references:There are no normative references in this document.
3.Principle:Free sterols/stanols and saponified steryl/stanol esters are derivatized to phytosterol trimethylsilyl(TMS) ethers and separated on a capillary gas chromatography column.
4.Reagents:Use only reagents of recognized analytical grade.
WARNING—Attention is drawn to regulations thatspecify the handling of hazardous substanceTechnical, organizational and personal safety measures shall be followed.A chemical fume hooshall be used for the work.
5.Weigh 92 g of sodium hydroxide (5.5) into a 1 000 ml volumetric flask(6.14).Add a stir bar (6.20) and750 ml of methanol (5.4). Place the flask on a magnetic stirrer (6.21) to disperse. Dilute to the markwith methanol, and invert to mix.The sodium hydroxide solution shall be stirred each time before usebecause it will settle out with time.
Accurately weigh 5,0 g of epicoprostanol (5.8) into a glass weighing scoop (6.8). Transfer theepicoprostanol to a 1 000 ml volumetric flask (6.14).Rinse the scoop with internal standard dissolvingsolvent (5.3) into the flask.Dilute to the mark with toluene or ethyl acetate and invert to mix. Theepicoprostanol lS solution of concentration 5 mg/ml is used for sterol/stanol concentrates,steryl/stanol ester concentrates and some dietary supplements.
Accurately weigh 2,0 g of epicoprostanol (5.8) into a glass weighing scoop (6.8). Transfer theepicoprostanol to a 1 000 ml volumetric flask (6.14).Rinse the scoop with internal standard dissolvingsolvent (5.3) into the flask. Dilute to the mark with toluene or ethyl acetate and invert to mix. Theepicoprostanol lS solution of concentration 2 mg/ml is used for analysis of foods and some dietarysupplements.ISO 23349 pdf download.