ISO 23293-2020 pdf free

04-19-2021 comment

ISO 23293-2020 pdf free.Milk-based infant formula powders —Quantification of whey protein contentby sodium dodecyl sulfate-capillarygel electrophoresis (sDS-CGE).
This document specifies a method for the determination of the whey to casein protein ratio, rangingfrom 20:80 to 80:20 in cow milk-based infant formula powders.
This method does not apply to the analysis of infant formulas containing hydrolysed protein or proteinsfrom other sources (e.g. plants or milk from other mammals).There are no normative references in this document.
For the purposes of this document, the following terms and definitions apply.
breast-milk substitute specially manufactured to satisfy, by itself, the nutritional requirements ofinfants during the first months of life up to the introduction of appropriate complementary feeding[SOURCE: Codex Standard 72-1981]
In sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE), proteins in infant formula samplesare denatured by the anionic surfactant SDS and reduced by $-mercaptoethanol. The SDS-bonded,electrically charged proteins migrate in an electrical field filled with a separation gel and are detectedby Uv at 220 nm.Caseins and whey proteins are separated as two distinct, non-overlapping groups ofpeaks where the ratio can be established based on integrated areas without the need for a calibrationcurve.In the calculation of whey protein content, 1,4 was used as the mass-to-area correction factor forwhey proteins versus caseins.During the analysis,unless otherwise stated,use only reagents of recognized analytical grade anddistilled or demineralized water or water of equivalent purity.
Data from the multi-laboratory study was obtained with chemicals from the SDS-MW kit 390953 fromBeckman Coulter/Sciex1).The SDS-MW gel separation buffer recipe described below(5.1) was tested ina separate study and found to be equivalent to the commercial SDS-MWgel separation buffer included inthe kit. One laboratory used home-made reagents (5.2 to 5.5).All laboratories used the 10 kDa internalprotein standard (5.6) and the SDS-MW size standard (5.7) from the kit. Use of equivalent standardsrequires prior verification.
5.1SDs-Mw gel separation buffer, tris(hydroxymethyl)aminomethane (Tris), boric acid,SDS,EDTA,glycerol and dextran 20001) .
Dissolve 0,726 6 g Tris base and 0,371 0 g boric acid in 9 ml water. Add 20 mg SDS, 1 g dextran2000,14,6 mg EDTA, and 1 ml glycerol.Mix thoroughly. Leave overnight to allow complete dextransolubilization.ISO 23293 pdf download.

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