AS 3896:2017 pdf free.Waters – Examination for Legionella spp. including Legionella pneumophila.
5 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
5.1 Culture media
The culture media shall he as follows:
(a) Buffered charcoal yeast extract agar (BCYE).
(b) Buffered charcoal yeast extract agar with MWY selective supplement (MWY) or buffered charcoal yeast extract agar with GVPC selective supplement (GVPC).
(c) Buffered charcoal yeast extract agar with BMPA selective supplement (BMPA) or buffered charcoal yeast extract agar with selective supplements (BCYE+AB).
(d) Buffered charcoal yeast extract medium without L-cysteinc (BCYE-Cys) or Blood Agar (BA).
(e) Diluent: sterile 0.1% peptone or sterile quarter strength Ringer’s solution.
NOTE: See Appendix B.
5.2 Reagents and identification systems
5.2.1 Acidbuffer
Acid buffer shall be prepared using the formulation and procedure.
NOTE: Sec Appendix B, Paragraph HI 1.
5.2.2 Specia tion/tvpmg system (s)
Speciation systems, using latex agglutination, direct or indirect immunofluorescence. mass spectrometric and molecular techniques are available for identifying known serogroups and/or genotypes of L. pneumnophila and other Legionella spp.
The laboratory shall ensure that the system or systems used are suitable for the laboratory’s requirements for identification/typing. Fitness for purpose may be demonstrated by reference to the manufacturer’s validation data or to evaluation studies published in the scientific literature.
5.3 Reference cultures
5.3.1 Positive cultures
Two positive cultures (Legionella pneumophila and a non—pneuinophila sp.) shall be used.
The following choice of the non-,nzeumnophila reference culture [see Clause 5.3.1(b) or
5.3.1(c)] is left to the discretion of the laboratory:
(a) Legionella pneumophila serogroup I WDCM 00107 or a culture traceable to this strain.
NOTE: WDCM—World Data Centre for Microorganisms.
(b) Legionella ,nicdadei (synonym Tallockia micdadei) ATCC 33218 or CIP 103882 or
NCTC 11371 or a culture traceable to one of these strains.
NOTES:
1 There is currently no WDCM culture reference for this strain.
2 ATCC—Amcrican Type Culture Collection.
3 CIP Collection of Institut Pasteur.
4 NCTC—National Collection of Type Cultures.
(c) Legionella bozemanue (synonym Fluoribacter ho:emanae) ATCC 3321 7 or NCTC 11368 or a culture traceable to one of these strains.
NOTE: There is currently no WDCM culture reference for this strain.AS 3896 pdf download.